PsyMuKB

PPI network (data source: BioGrid)

A gene is determined as “expressed” when its expression values are above the pre-determined cutoff, which is the mean normalized expression(base 2 log of (TPM+1)) +1 in all brain tissue from GTEx v7 or BrainSpan data.

A protein is determined as “expressed” when its expression values are above the pre-determined cutoff, which is the normalized expression (base 10 log of iBAQ intensity) ≥0.5 in at least one brain tissue from ProteomicsDB data. Otherwise, a gene or protein is determined as either with below-cutoff expressions or no available data.

	The queried gene/protein
	The interacting partner is expressed at mRNA-level
	The interacting partner is expressed at protein-level
	The interacting partner is expressed at both mRNA and protein level
	The interacting partner is with either below-cutoff expressions or no expression data at both mRNA and protein level
	The interacting partner is hit by coding DNMs
	Physical interaction between queried gene/protein and its partner
	Physical interaction between two interacting partners

Symbol A	GeneA coding DNM count	Symbol B	GeneB coding DNM count	Interaction detection method	Publication	Evidence
PRKN	1	UBE2N	1	pull down, pull down, pull down, affinity chromatography technology, affinity chromatography technology, pull down, pull down, pull down	Joch M (2007), Olzmann JA (2007), Chaugule VK (2011), Sha D (2010), Lim GG (2013), Durcan TM (2011), McKeon JE (2014), Sha D (2010)	8
UBE2V2	0	UBC	7	x-ray crystallography, pull down, x-ray crystallography, x-ray crystallography, pull down	McKenna S (2003), McKenna S (2003), McKenna S (2003), Lewis MJ (2006), McKenna S (2001)	5
TRIM5	4	UBC	7	enzymatic study	Pertel T (2011)	1
HLTF	7	UBC	7	enzymatic study	Masuda Y (2012)	1
LRSAM1	2	RNF111	2	two hybrid	Woodsmith J (2012)	1
TRAF6	0	UBC	7	affinity chromatography technology, affinity chromatography technology, enzymatic study, enzymatic study, enzymatic study, affinity chromatography technology, enzymatic study	Muroi M (2011), Park S (2013), Sun L (2004), Kanayama A (2004), Sakamoto H (2015), Yu J (2016), Middleton AJ (2017)	7
UBE2V1	0	TRIM5	4	two hybrid	Markson G (2009)	1
UBC	7	MIB1	5	affinity chromatography technology	Matsumoto M (2005)	1
OTUB1	0	UBE2V2	0	pull down	Sato Y (2012)	1
BRCA1	7	XIAP	0	affinity chromatography technology, pull down	Martin SA (2005), Martin SA (2005)	2
UBE2N	1	TRIM27	2	two hybrid, affinity chromatography technology	Napolitano LM (2011), Napolitano LM (2011)	2
UBE2V1	0	MID1	1	two hybrid	Markson G (2009)	1
UBE2N	1	BIRC3	1	two hybrid, pull down, two hybrid	van Wijk SJ (2009), Wu CJ (2006), Schimmack G (2017)	3
TRIM32	2	UBE2V2	0	two hybrid, two hybrid, affinity chromatography technology	Napolitano LM (2011), Napolitano LM (2011), Napolitano LM (2011)	3
AMFR	2	UBC	7	pull down, pull down, imaging technique, affinity chromatography technology, enzymatic study, x-ray crystallography, pull down, x-ray crystallography, pull down, enzymatic study, enzymatic study, pull down, affinity chromatography technology	Zhong X (2004), Yang H (2010), Ying Z (2009), Jo Y (2011), Ye Y (2003), Xu Y (2012), Liu S (2012), Liu S (2012), Xu Y (2012), Morito D (2008), Flierman D (2006), Liu W (2014), Fang S (2001)	13
TRAF6	0	TP53	0	enzymatic study, affinity chromatography technology	Zhang X (2016), Zhang X (2016)	2
TRIM25	5	UBA1	1	affinity chromatography technology	Pauli EK (2014)	1
TRAF2	1	UBC	7	enzymatic study, enzymatic study	Liu J (2011), Kanayama A (2004)	2
BIRC3	1	XIAP	0	affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Rajalingam K (2006), Rajalingam K (2006), Yang W (2014), Yang W (2014)	4
UBE2V2	0	XIAP	0	two hybrid	Markson G (2009)	1
UBE2V2	0	TRIM5	4	two hybrid	Markson G (2009)	1
STUB1	4	TRAF2	1	affinity chromatography technology	Jang KW (2011)	1
UBE2V1	0	UBE2V2	0	biochemical	Havugimana PC (2012)	1
UBE2V1	0	STUB1	4	pull down, pull down, pull down, biochemical, affinity chromatography technology, pull down	Zhang M (2005), Yang M (2011), Windheim M (2008), Havugimana PC (2012), Zhang M (2005), Zhang M (2005)	6
ARIH2	4	TP53	0	two hybrid, affinity chromatography technology, pull down	Stelzl U (2005), Bae S (2012), Bae S (2012)	3
XIAP	0	STUB1	4	pull down	Dogan T (2008)	1
UBC	7	STUB1	4	affinity chromatography technology, affinity chromatography technology, enzymatic study, enzymatic study	Connell P (2001), Connell P (2001), Windheim M (2008), Zhang M (2005)	4
TP53	0	UBC	7	affinity chromatography technology, affinity chromatography technology	Ayroldi E (2014), Ke JY (2014)	2
TRIM25	5	SH3RF1	1	affinity chromatography technology	Choudhury NR (2017)	1
TRAF2	1	UBE2V2	0	pull down	Sasai M (2010)	1
ARIH2	4	TRIM27	2	two hybrid	Woodsmith J (2012)	1
HLTF	7	SHPRH	7	affinity chromatography technology	Lin JR (2011)	1
CNEP1R1	0	UBE2N	1	two hybrid, pull down, pull down, affinity chromatography technology, affinity chromatography technology	Chen J (2015), Chen J (2015), Chen J (2015), Chen J (2015), Chen J (2015)	5
TRAF2	1	XIAP	0	affinity chromatography technology	Vince JE (2009)	1
UBE2N	1	ARIH2	4	two hybrid, affinity chromatography technology, pull down	Marteijn JA (2009), Marteijn JA (2009), Marteijn JA (2009)	3
UBE2N	1	ZNRF2	0	two hybrid, two hybrid, affinity chromatography technology, affinity chromatography technology, two hybrid, pull down, affinity chromatography technology	van Wijk SJ (2009), Plans V (2006), Hoxhaj G (2012), Hoxhaj G (2012), Scheper J (2009), Hoxhaj G (2012), Boldt K (2016)	7
BRCA1	7	HERC2	9	affinity chromatography technology, affinity chromatography technology, enzymatic study, affinity chromatography technology	Wu W (2010), Wu W (2010), Wu W (2010), Peng Y (2015)	4
TRAF6	0	XIAP	0	affinity chromatography technology, two hybrid, imaging technique	Sanna MG (2002), Woodsmith J (2012), Chen TC (2014)	3
STUB1	4	TRAF6	0	pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, enzymatic study, pull down	Yang M (2011), Yang M (2011), Wang S (2013), Li S (2014), Li S (2014), Li S (2014), Li S (2014)	7
RC3H2	5	UBC	7	enzymatic study	Zhang Q (2015)	1
UBC	7	HERC2	9	affinity chromatography technology, pull down	Rendtlew Danielsen J (2012), Zhang X (2017)	2
UBE2N	1	MARCH7	0	two hybrid, two hybrid, pull down, two hybrid	Markson G (2009), Flach K (2014), Flach K (2014), Nathan JA (2008)	4
BRCA1	7	UBA1	1	affinity chromatography technology	Ertych N (2016)	1
TRIM32	2	TP53	0	affinity chromatography technology, affinity chromatography technology	Liu J (2014), Liu J (2014)	2
RNF111	2	MARCH7	0	two hybrid	Woodsmith J (2012)	1
TP53	0	STUB1	4	affinity chromatography technology, enzymatic study, pull down, affinity chromatography technology, enzymatic study, enzymatic study, affinity chromatography technology, enzymatic study, enzymatic study, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, enzymatic study, enzymatic study, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Muller P (2008), Stankiewicz M (2010), Tripathi V (2007), Tripathi V (2007), Esser C (2005), Lim JH (2011), Wang J (2011), Burch L (2004), Landre V (2013), Horikawa I (2014), Horikawa I (2014), Horikawa I (2014), Fraser JA (2015), Narayan V (2015), Yue X (2016), Parrales A (2016), Parrales A (2016)	17
MARCH7	0	UBC	7	enzymatic study	Flierman D (2006)	1
HLTF	7	UBE2N	1	affinity chromatography technology, pull down, pull down, enzymatic study	Unk I (2008), Unk I (2008), Masuda Y (2012), Masuda Y (2012)	4
RNF168	0	HERC2	9	affinity chromatography technology	Bekker-Jensen S (2010)	1
UBC	7	UBA1	1	affinity chromatography technology, unspecified method, x-ray crystallography, biochemical, affinity chromatography technology, pull down	Nicassio F (2007), Zhao B (2012), Schaefer A (2014), Schaefer A (2014), Bish RA (2007), Zhang X (2017)	6
TRAF2	1	TRAF6	0	two hybrid, affinity chromatography technology, affinity chromatography technology, two hybrid, two hybrid, two hybrid, affinity chromatography technology	Rual JF (2005), Li S (2011), Davies CC (2005), Relaix F (1998), Rolland T (2014), Yu H (2011), Huttlin EL (2017)	7
TRIM25	5	AMFR	2	enzymatic study, pull down	Wang Y (2014), Wang Y (2014)	2
TNF	0	UBE2N	1	genetic interference, affinity chromatography technology	Deng L (2000), Fritsch J (2014)	2
RNF8	2	UBC	7	enzymatic study, enzymatic study, enzymatic study	Feng L (2012), Hodge CD (2015), Ho YK (2015)	3
CHFR	5	HLTF	7	affinity chromatography technology, pull down, enzymatic study, affinity chromatography technology	Kim JM (2010), Kim JM (2010), Kim JM (2010), Oh YM (2009)	4
TRAF6	0	BIRC3	1	affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Mao AP (2010), Goncharov T (2013), Harikumar KB (2014), Harikumar KB (2014)	4
UBE2V1	0	RNF111	2	two hybrid	Markson G (2009)	1
TRIM25	5	STUB1	4	affinity chromatography technology	Choudhury NR (2017)	1
UBE2N	1	MIB1	5	two hybrid, pull down	van Wijk SJ (2009), Li S (2011)	2
OTUB1	0	UBE2N	1	affinity chromatography technology, affinity chromatography technology, pull down, pull down, pull down, affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, pull down	Sowa ME (2009), Nakada S (2010), Nakada S (2010), Sato Y (2012), Sato Y (2012), Sato Y (2012), Wiener R (2012), Herhaus L (2013), Herhaus L (2015), Scholz CC (2016), Boldt K (2016), Weber E (2017)	12
BIRC3	1	UBE2V1	0	pull down, pull down	Bertrand MJ (2008), Wu CJ (2006)	2
PRKN	1	STUB1	4	affinity chromatography technology, affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology	Imai Y (2002), Imai Y (2002), Imai Y (2002), Imai Y (2003), Zhu X (2017)	5
UBE2V1	0	ZNRF1	1	two hybrid	Markson G (2009)	1
TRIM25	5	CHFR	5	affinity chromatography technology	Choudhury NR (2017)	1
TMEM189	0	UBC	7	pull down	Duex JE (2010)	1
OTUB1	0	EPM2A	1	affinity chromatography technology	Scholz CC (2016)	1
TRIM32	2	UBC	7	enzymatic study, pull down	Streich FC (2013), Zhang X (2017)	2
TNF	0	TRAF2	1	affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Micheau O (2003), Kuai J (2004), Kuai J (2004), Haas TL (2009), Ea CK (2006), Fritsch J (2014), Yamaguchi N (2015)	7
TRIM11	1	UBE2N	1	two hybrid, two hybrid, affinity chromatography technology, affinity chromatography technology	Napolitano LM (2011), Napolitano LM (2011), Napolitano LM (2011), Boldt K (2016)	4
UBE2N	1	HERC2	9	affinity chromatography technology, pull down	Bekker-Jensen S (2010), Bai B (2016)	2
UBE2N	1	STUB1	4	pull down, pull down, pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, pull down, affinity chromatography technology, pull down, pull down, pull down	Zhang M (2005), Graf C (2010), Soss SE (2011), Huen MS (2008), Lim GG (2013), Taipale M (2014), Taipale M (2014), Zhu X (2014), Zhang M (2005), Zhang M (2005), Kim HT (2007), Blessing NA (2017)	12
UBE2N	1	UBA1	1	affinity chromatography technology, enzymatic study, enzymatic study, affinity chromatography technology	Huen MS (2008), Wu PY (2003), Wauer T (2015), Boldt K (2016)	4
ZNRF2	0	ZNRF1	1	affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Hoxhaj G (2012), Hoxhaj G (2012), Hoxhaj G (2012), Hoxhaj G (2012)	4
TRIM27	2	MID2	1	two hybrid	Rolland T (2014)	1
UBE2N	1	RC3H2	5	two hybrid, pull down	Markson G (2009), Zhang Q (2015)	2
UBE2V1	0	UBC	7	pull down	McKenna S (2003)	1
SHPRH	7	UBE2V2	0	affinity chromatography technology, pull down	Unk I (2006), Unk I (2006)	2
UBE2N	1	XIAP	0	two hybrid, pull down	Markson G (2009), Markson G (2009)	2
HLTF	7	UBE2V2	0	affinity chromatography technology, pull down, pull down	Unk I (2008), Unk I (2008), Masuda Y (2012)	3
BIRC3	1	TRAF2	1	two hybrid, pull down, affinity chromatography technology, affinity chromatography technology, pull down, affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology, x-ray crystallography, pull down, pull down, affinity chromatography technology, affinity chromatography technology, two hybrid, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, two hybrid, affinity chromatography technology, enzymatic study, imaging technique, affinity chromatography technology	Uren AG (1996), Roy N (1997), Yoneda T (2000), Yoneda T (2000), Li X (2002), Behrends C (2010), Samuel T (2006), Vince JE (2009), Darding M (2011), Zheng C (2010), Zheng C (2010), Zheng C (2010), Zheng C (2010), Zheng C (2010), Rothe M (1995), Varfolomeev E (2006), Choudhary S (2013), Goncharov T (2013), Wang J (2011), Harikumar KB (2014), Shen RR (2012), Chen TC (2014), Akhtar N (2016)	23
MID2	1	BRCA1	7	two hybrid	Hill SJ (2014)	1
BRCA1	7	UBE2N	1	two hybrid, x-ray crystallography, pull down, two hybrid, pull down	Christensen DE (2009), Christensen DE (2009), Christensen DE (2009), Christensen DE (2007), David Y (2011)	5
TRIM32	2	MID2	1	two hybrid	Reymond A (2001)	1
UBE2N	1	ZNRF1	1	two hybrid, two hybrid, affinity chromatography technology, affinity chromatography technology, pull down, pull down, affinity chromatography technology	van Wijk SJ (2009), Markson G (2009), Hoxhaj G (2012), Hoxhaj G (2012), Markson G (2009), Hoxhaj G (2012), Boldt K (2016)	7
TRIM32	2	TRIM27	2	two hybrid, two hybrid	Reymond A (2001), Woodsmith J (2012)	2
UBE2N	1	CHFR	5	two hybrid, pull down	Markson G (2009), Liu C (2013)	2
STUB1	4	UBE2V2	0	biochemical	Havugimana PC (2012)	1
RNF11	0	RNF168	0	two hybrid	Colland F (2004)	1
UBE2N	1	MID2	1	two hybrid, two hybrid, two hybrid, affinity chromatography technology	van Wijk SJ (2009), Napolitano LM (2011), Napolitano LM (2011), Napolitano LM (2011)	4
UBE2N	1	RNF11	0	two hybrid, two hybrid, two hybrid, two hybrid, affinity chromatography technology	van Wijk SJ (2009), Markson G (2009), Scheper J (2009), Colland F (2004), Poulsen SL (2013)	5
AURKA	1	UBE2N	1	pull down, affinity chromatography technology, enzymatic study	Ewart-Toland A (2003), Ewart-Toland A (2003), Briassouli P (2006)	3
RC3H2	5	UBE2V1	0	pull down	Zhang Q (2015)	1
PRKN	1	AURKA	1	affinity chromatography technology	Lee SB (2015)	1
UBC	7	UBE2N	1	affinity chromatography technology, enzymatic study, enzymatic study, x-ray crystallography, enzymatic study, enzymatic study, affinity chromatography technology, enzymatic study, enzymatic study, enzymatic study, enzymatic study, pull down	Matsumoto M (2005), Scheper J (2010), Wu PY (2003), McKenna S (2003), Wiener R (2012), Ye Y (2003), Bish RA (2007), Juang YC (2012), Pastushok L (2007), Singh RK (2012), McKenna S (2001), McKenna S (2001)	12
UBE2N	1	RNF152	0	two hybrid, pull down	van Wijk SJ (2009), Deng L (2015)	2
TP53	0	UBE2N	1	pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, enzymatic study	Laine A (2006), Laine A (2006), Laine A (2006), Topisirovic I (2009), Topisirovic I (2009)	5
ISG15	0	TRIM25	5	affinity chromatography technology, affinity chromatography technology	Zou W (2007), Zou W (2007)	2
UBE2N	1	RNF103	1	two hybrid, two hybrid, two hybrid	van Wijk SJ (2009), Plans V (2006), Scheper J (2009)	3
TRIM27	2	TRIM11	1	two hybrid	Reymond A (2001)	1
RNF111	2	UBC	7	enzymatic study	Wright JD (2016)	1
XIAP	0	UBC	7	x-ray crystallography, pull down, pull down, pull down	Tse MK (2011), Gyrd-Hansen M (2008), Gyrd-Hansen M (2008), Nakatani Y (2012)	4
UBE2N	1	RNF8	2	two hybrid, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, pull down, two hybrid, affinity chromatography technology, two hybrid, affinity chromatography technology, two hybrid, affinity chromatography technology, pull down, pull down, fluorescent resonance energy transfe, pull down, two hybrid	Markson G (2009), Bekker-Jensen S (2010), Lok GT (2011), Lok GT (2011), Campbell SJ (2012), Plans V (2006), Plans V (2006), Huen MS (2008), Zhang X (2013), Guerra-Rebollo M (2012), Zhao H (2014), Hodge CD (2015), Ho YK (2015), Weber E (2017), Weber E (2017), Schimmack G (2017)	16
TRIM25	5	UBE2V1	0	pull down	Sanchez JG (2016)	1
UBE2N	1	TRIM5	4	two hybrid, pull down, x-ray crystallography	Markson G (2009), Yudina Z (2015), Yudina Z (2015)	3
TRAF2	1	PRKN	1	affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Henn IH (2007), Henn IH (2007), Um JW (2012), Im E (2015)	4
RNF165	0	UBC	7	enzymatic study, x-ray crystallography, pull down	Wright JD (2016), Wright JD (2016), Wright JD (2016)	3
PRKN	1	TP53	0	pull down, enzymatic study	Jung YY (2017), Jung YY (2017)	2
MID1	1	MID2	1	two hybrid, two hybrid, two hybrid, two hybrid, affinity chromatography technology, affinity chromatography technology, two hybrid, two hybrid, two hybrid, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Reymond A (2001), Reymond A (2001), Short KM (2002), Short KM (2002), Short KM (2002), Short KM (2002), Short KM (2006), Short KM (2006), Rolland T (2014), Gholkar AA (2016), Gholkar AA (2016), Gholkar AA (2016), Gholkar AA (2016)	13
HERC2	9	TP53	0	affinity chromatography technology, affinity chromatography technology, pull down, pull down	Cubillos-Rojas M (2014), Cubillos-Rojas M (2014), Cubillos-Rojas M (2014), Bai B (2016)	4
TRIM50	1	UBE2N	1	two hybrid, two hybrid	Napolitano LM (2011), Napolitano LM (2011)	2
ZNRF1	1	UBC	7	enzymatic study	Hoxhaj G (2012)	1
TRAF6	0	UBE2V1	0	pull down, pull down, pull down, pull down, pull down, pull down, pull down, pull down, pull down, pull down	Deng L (2000), Lamothe B (2007), Kanayama A (2004), Shi CS (2010), Xia ZP (2009), Yang WL (2009), Murata H (2013), Ning S (2008), Sun L (2004), Lin Y (2014)	10
TRIM25	5	TMEM189	0	affinity chromatography technology	Choudhury NR (2017)	1
UBE2V1	0	ARIH2	4	two hybrid	Markson G (2009)	1
RC3H2	5	UBE2V2	0	pull down	Zhang Q (2015)	1
TRIM25	5	RNF8	2	affinity chromatography technology	Choudhury NR (2017)	1
BRCA1	7	TRIM25	5	affinity chromatography technology	Ertych N (2016)	1
SH3RF1	1	UBE2N	1	pull down, pull down	Votteler J (2009), Tuvia S (2007)	2
TRAF6	0	UBE2N	1	genetic interference, pull down, two hybrid, biochemical, two hybrid, x-ray crystallography, two hybrid, enzymatic study, x-ray crystallography, pull down, pull down, pull down, pull down, pull down, pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, pull down, pull down, two hybrid, two hybrid	Deng L (2000), Deng L (2000), Markson G (2009), Yin Q (2009), Dynek JN (2010), Yin Q (2009), Yin Q (2009), Petroski MD (2007), Mercier P (2007), Lamothe B (2008), Kanayama A (2004), Murata H (2013), Ning S (2008), Markson G (2009), Lin Y (2014), Chen J (2015), Zhang J (2016), Boldt K (2016), Rezaeian AH (2017), Xing J (2016), Schimmack G (2017), Schimmack G (2017)	22
UBE2N	1	UBE2V1	0	biochemical, pull down, pull down, pull down, two hybrid, two hybrid, pull down, affinity chromatography technology, two hybrid, two hybrid, affinity chromatography technology, two hybrid, biochemical, pull down, pull down, pull down, biochemical, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Deng L (2000), Deng L (2000), Zhang M (2005), Andersen PL (2005), Andersen PL (2005), Scheper J (2010), Scheper J (2010), Scheper J (2010), Wooff J (2004), Plans V (2006), Huen MS (2008), Huen MS (2008), Havugimana PC (2012), Bohgaki M (2008), Petroski MD (2007), Sawasaki T (2008), Wan C (2015), Chen J (2015), Boldt K (2016), Zhang Y (2018)	20
TP53	0	UBE2V2	0	two hybrid	Daakour S (2016)	1
AURKA	1	CHFR	5	affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Privette LM (2008), Oh YM (2009), Oh YM (2009)	3
BRCA1	7	TP53	0	affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, pull down, affinity chromatography technology, pull down, biochemical, pull down, pull down, affinity chromatography technology, pull down, pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, pull down	Abramovitch S (2004), Ouchi T (1998), Dong Y (2003), Dong Y (2003), Chai YL (1999), Chai YL (1999), Zhang H (1998), Dong Y (2003), Chen Y (2006), Quaresima B (2006), Zhang H (1998), Zhang H (1998), Mark WY (2005), Jin S (2009), Jiang J (2011), Rasti M (2015), Rasti M (2015)	17
UBC	7	BRCA1	7	pull down, enzymatic study	Sobhian B (2007), Zhao GY (2007)	2
PRKN	1	UBE2V1	0	pull down, pull down, pull down, affinity chromatography technology	Chaugule VK (2011), Durcan TM (2011), Sha D (2010), Sha D (2010)	4
UBE2V2	0	ZNRF1	1	two hybrid	Markson G (2009)	1
UBE2N	1	RNF111	2	affinity chromatography technology, pull down	Poulsen SL (2013), Wright JD (2016)	2
UBE2V1	0	TRIM32	2	two hybrid, two hybrid, two hybrid, affinity chromatography technology	Markson G (2009), Napolitano LM (2011), Napolitano LM (2011), Napolitano LM (2011)	4
TP53	0	AURKA	1	two hybrid, affinity chromatography technology, affinity chromatography technology, pull down, genetic interference, pull down, pull down, affinity chromatography technology, affinity chromatography technology, enzymatic study, affinity chromatography technology, affinity chromatography technology	Chen SS (2002), Chen SS (2002), Chen SS (2002), Chen SS (2002), Chen SS (2002), Katayama H (2004), Katayama H (2004), Katayama H (2004), Katayama H (2004), Hsueh KW (2013), Huttlin EL (2015), Huttlin EL (2017)	12
AURKA	1	UBA1	1	biochemical	Wan C (2015)	1
BIRC3	1	TRIM32	2	affinity chromatography technology	Ryu YS (2011)	1
UBE2N	1	MID1	1	two hybrid, two hybrid, two hybrid, affinity chromatography technology	Markson G (2009), Napolitano LM (2011), Napolitano LM (2011), Napolitano LM (2011)	4
TMEM189	0	UBE2N	1	pull down, two hybrid	Andersen PL (2005), Andersen PL (2005)	2
OTUB1	0	STUB1	4	affinity chromatography technology	Scholz CC (2016)	1
RNF4	1	UBE2N	1	pull down, x-ray crystallography	Branigan E (2015), Branigan E (2015)	2
TRIM25	5	RNF4	1	affinity chromatography technology	Choudhury NR (2017)	1
PRKN	1	UBC	7	enzymatic study, imaging technique, enzymatic study, fluorescent resonance energy transfe, pull down, pull down, pull down, pull down, x-ray crystallography, imaging technique, affinity chromatography technology, pull down, imaging technique, affinity chromatography technology, enzymatic study, enzymatic study, pull down, x-ray crystallography, enzymatic study	Kondapalli C (2012), Kane LA (2014), Kane LA (2014), Koyano F (2014), Zheng X (2013), Shiba-Fukushima K (2014), Shiba-Fukushima K (2014), Kumar A (2015), Kumar A (2015), Okatsu K (2015), Okatsu K (2015), Okatsu K (2015), Yamano K (2015), Fiesel FC (2015), Ordureau A (2015), Kazlauskaite A (2015), Kazlauskaite A (2015), Kumar A (2017), Kliza K (2017)	19
UBE2N	1	EPM2A	1	affinity chromatography technology, affinity chromatography technology	Sanchez-Martin P (2015), Sanchez-Martin P (2015)	2
RNF8	2	UBE2V2	0	pull down	Campbell SJ (2012)	1
TRIM27	2	TP53	0	affinity chromatography technology, pull down, enzymatic study	Chu Y (2011), Chu Y (2011), Chu Y (2011)	3
TRIM25	5	RC3H2	5	affinity chromatography technology	Choudhury NR (2017)	1
TRAF2	1	UBE2V1	0	pull down	Deng L (2000)	1
RHNO1	1	UBE2N	1	affinity chromatography technology, affinity chromatography technology	Cotta-Ramusino C (2011), Cotta-Ramusino C (2011)	2
TRAF2	1	UBE2N	1	genetic interference, pull down, affinity chromatography technology, affinity chromatography technology, pull down, pull down, pull down, pull down, pull down	Deng L (2000), Deng L (2000), Liu J (2011), Liu J (2011), Liu J (2011), Alvarez SE (2010), Sasai M (2010), Zhou AY (2013), Shen RR (2012)	9
XIAP	0	TRIM32	2	affinity chromatography technology, affinity chromatography technology, enzymatic study	Ryu YS (2011), Ryu YS (2011), Ryu YS (2011)	3
UBE2N	1	TRIM25	5	two hybrid, pull down, x-ray crystallography, affinity chromatography technology	Markson G (2009), Sanchez JG (2016), Sanchez JG (2016), Choudhury NR (2017)	4
ARIH2	4	UBC	7	enzymatic study	Marteijn JA (2009)	1
OTUB1	0	UBC	7	enzymatic study, enzymatic study, x-ray crystallography, unspecified method, pull down, enzymatic study, enzymatic study, enzymatic study, affinity chromatography technology, enzymatic study, pull down, enzymatic study, x-ray crystallography, enzymatic study, pull down, pull down, enzymatic study, enzymatic study, enzymatic study, affinity chromatography technology, enzymatic study, enzymatic study, enzymatic study, enzymatic study, pull down, pull down	Wang T (2009), Bremm A (2010), Juang YC (2012), Ernst A (2013), Ernst A (2013), Nakasone MA (2013), Wiener R (2013), Edelmann MJ (2009), Bish RA (2007), Mevissen TE (2013), Juang YC (2012), Herhaus L (2013), Ernst A (2013), Herhaus L (2015), Herhaus L (2015), Herhaus L (2015), Wauer T (2015), Michel MA (2015), Kristariyanto YA (2015), Scholz CC (2016), Scholz CC (2016), Altun M (2015), Flierman D (2016), Castaneda CA (2016), Zhang X (2017), Kwasna D (2018)	26
MID1	1	UBC	7	affinity chromatography technology, affinity chromatography technology	Trockenbacher A (2001), Trockenbacher A (2001)	2
UBE2N	1	LRSAM1	2	two hybrid, two hybrid, two hybrid	van Wijk SJ (2009), Markson G (2009), Hakonen JE (2017)	3
TRIM5	4	TRIM32	2	two hybrid	Woodsmith J (2012)	1
UBE2V1	0	XIAP	0	two hybrid	Markson G (2009)	1
UBE2N	1	UBE2N	1	enzymatic study, pull down, enzymatic study	David Y (2010), David Y (2010), McKenna S (2001)	3
RNF168	0	UBE2N	1	affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Nakada S (2010), Stewart GS (2009), Zhang X (2013), Zhao H (2014), Stewart GS (2009)	5
TP53	0	OTUB1	0	affinity chromatography technology, affinity chromatography technology, pull down, affinity chromatography technology, enzymatic study	Sun XX (2011), Sun XX (2011), Sun XX (2011), Li Y (2014), Fernandez-Majada V (2016)	5
BIRC3	1	UBA1	1	affinity chromatography technology	Goncharov T (2013)	1
TRAF6	0	TRIM25	5	two hybrid	Woodsmith J (2012)	1
OTUB1	0	TRAF6	0	affinity chromatography technology, enzymatic study	Li S (2010), Bremm A (2010)	2
BIRC3	1	OTUB1	0	affinity chromatography technology	Goncharov T (2013)	1
AURKA	1	BRCA1	7	affinity chromatography technology, pull down, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, enzymatic study, affinity chromatography technology	Ouchi M (2004), Ouchi M (2004), Maxwell CA (2011), Maxwell CA (2011), Ouchi M (2004), Ouchi M (2004), Ertych N (2016)	7
RNF8	2	HERC2	9	affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, two hybrid, affinity chromatography technology	Bekker-Jensen S (2010), Bekker-Jensen S (2010), Bekker-Jensen S (2010), Rendtlew Danielsen J (2012), Guerra-Rebollo M (2012), Zhang Z (2014)	6
BIRC3	1	UBC	7	pull down, pull down, enzymatic study	Gyrd-Hansen M (2008), Nakatani Y (2012), Sakamoto H (2015)	3
ISG15	0	UBE2N	1	affinity chromatography technology, affinity chromatography technology, affinity chromatography technology, affinity chromatography technology	Zhao C (2005), Bade VN (2012), Bade VN (2012), Takeuchi T (2005)	4
SHPRH	7	UBE2N	1	affinity chromatography technology, pull down	Unk I (2006), Unk I (2006)	2
TRIM25	5	HERC2	9	affinity chromatography technology	Choudhury NR (2017)	1
TRIM32	2	UBE2N	1	two hybrid, affinity chromatography technology, two hybrid, two hybrid, two hybrid, two hybrid, affinity chromatography technology, affinity chromatography technology	Saccone V (2008), Saccone V (2008), van Wijk SJ (2009), Markson G (2009), Napolitano LM (2011), Napolitano LM (2011), Napolitano LM (2011), Boldt K (2016)	8
TRIM25	5	UBC	7	enzymatic study	Sanchez JG (2016)	1
UBE2N	1	RNF165	0	two hybrid, pull down	van Wijk SJ (2009), Wright JD (2016)	2
STUB1	4	EPM2A	1	affinity chromatography technology	Sengupta S (2011)	1
RNF11	0	UBC	7	two hybrid, two hybrid	Li H (2004), Colland F (2004)	2
RNF11	0	UBE2V2	0	affinity chromatography technology	Poulsen SL (2013)	1
UBC	7	TRIM27	2	affinity chromatography technology	Matsumoto M (2005)	1
UBE2N	1	AMFR	2	two hybrid, two hybrid	van Wijk SJ (2009), Markson G (2009)	2
TNF	0	RNF8	2	affinity chromatography technology	Fritsch J (2014)	1
OTUB1	0	AURKA	1	enzymatic study	Min M (2015)	1
HLTF	7	BRCA1	7	affinity chromatography technology	Tian F (2013)	1
UBE2V2	0	UBE2N	1	pull down, two hybrid, x-ray crystallography, two hybrid, two hybrid, two hybrid, pull down, pull down, x-ray crystallography, affinity chromatography technology, pull down, two hybrid, pull down, pull down, affinity chromatography technology, two hybrid, pull down, biochemical, x-ray crystallography, pull down, affinity chromatography technology, pull down, x-ray crystallography, biochemical, x-ray crystallography, affinity chromatography technology	Andersen PL (2005), Andersen PL (2005), Markin CJ (2010), Wooff J (2004), Wooff J (2004), Plans V (2006), Wu PY (2003), McKenna S (2003), McKenna S (2003), Zou W (2005), Takeuchi T (2005), Pastushok L (2005), Pastushok L (2005), Pastushok L (2005), Huen MS (2008), Huen MS (2008), Ye Y (2003), Havugimana PC (2012), Moraes TF (2001), Pastushok L (2007), Zou W (2005), McKenna S (2001), Hodge CD (2015), Wan C (2015), Branigan E (2015), Boldt K (2016)	26
RNF168	0	UBC	7	pull down, pull down, pull down, pull down, pull down	Panier S (2012), Feng L (2012), Thorslund T (2015), Luijsterburg MS (2017), Luijsterburg MS (2017)	5
RNF4	1	UBC	7	enzymatic study, enzymatic study	Groocock LM (2014), Branigan E (2015)	2
ZNRF2	0	UBC	7	enzymatic study	Hoxhaj G (2012)	1
TNF	0	UBC	7	affinity chromatography technology, affinity chromatography technology	Haas TL (2009), Fritsch J (2014)	2